Journal: World Journal of Surgical Oncology
Article Title: Analysis of the proliferative role and prognostic value of GPR173 in gastric cancer
doi: 10.1186/s12957-026-04274-x
Figure Lengend Snippet: Impact of GPR173 on gastric cancer cell proliferation in vitro and in vivo. A Relative mRNA expression of GPR173 in 10 pairs of gastric cancer tissues (Tumor) and adjacent Non-tumor tissues (Non-tumor) was measured by RT-qPCR. B Protein expression level of GPR173 in the human Non-tumor gastric mucosal epithelial cell line (GES-1) and various gastric cancer cell lines was detected by Western blot. C Western blot analysis validating the knockdown efficiency of GPR173 in MKN-45 cells and overexpression efficiency in AGS cells at the protein level. GAPDH served as the loading control. D The relative mRNA expression of GPR173 in MKN-45 cells transfected with different siRNAs (si-1, si-2, si-3) compared to negative control (NC) was determined by RT-qPCR. E The relative mRNA expression of GPR173 in AGS cells transfected with GPR173 overexpression plasmid (OE) compared to empty vector (Vector) was confirmed by RT-qPCR. F , H A colony formation assay and its quantification showed that knockdown of GPR173 inhibited the colony-forming ability of MKN-45 cells. G , H A colony formation assay and its quantification indicated that overexpression of GPR173 (OE) enhanced the colony-forming ability of AGS cells. I , K An EdU incorporation assay and its quantification revealed that knockdown of GPR173 suppressed the proliferation of MKN-45 cells. J , K An EdU incorporation assay and its quantification demonstrated that overexpression of GPR173 promoted the proliferation of AGS cells. L A CCK-8 assay showed that GPR173 knockdown inhibited the viability of MKN-45 cells. M A CCK-8 assay indicated that GPR173 overexpression enhanced the viability of AGS cells. N Representative images of subcutaneous xenograft tumors in nude mice injected with GPR173-overexpressing AGS cells (OE-GPR173) or control cells (Vector). O Quantitative comparison of tumor weights from each group at the experimental endpoint. P Tumor growth curves showing changes in tumor volume over time for each group. Q Representative images of IHC staining for GPR173 and the proliferation marker Ki-67 in subcutaneous xenograft tumors. Data are presented as the mean ± standard deviation (SD). Statistical significance was determined using Student’s t-test for comparisons between two groups A , D - O and one-way ANOVA for multiple comparisons. Tumor growth curves P were analyzed using two-way ANOVA
Article Snippet: The human Non-tumor gastric epithelial cell line GES-1 and human GC cell lines (MKN-45, HGC-27, SNU-216, MKN-73, AGS) were obtained from Genechem (Shanghai, China) or the Chinese Academy of Sciences Cell Bank (Shanghai, China).
Techniques: In Vitro, In Vivo, Expressing, Quantitative RT-PCR, Western Blot, Knockdown, Over Expression, Control, Transfection, Negative Control, Plasmid Preparation, Colony Assay, CCK-8 Assay, Injection, Comparison, Immunohistochemistry, Marker, Standard Deviation
